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Shinshu University School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

piggyBac Transposon System Modification of Primary Human T Cells

Sunandan Saha^1,2, Yozo Nakazawa³, Leslie E. Huye^4,5, Joseph E. Doherty^2,6, Daniel L. Galvan², Cliona M. Rooney^4,5,7, Matthew H. Wilson^1,2,4,8

¹Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , ²Department of Medicine, Division of Nephrology, Baylor College of Medicine , ³Department of Immunology and Pathology, Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy, Baylor College of Medicine , ⁵Department of Pediatrics, Baylor College of Medicine , ⁶Program in Cell and Molecular Biology, Baylor College of Medicine , ⁷Department of Molecular Virology and Microbiology, Baylor College of Medicine , ⁸Michael E. DeBakey VA Medical Center

We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.

Enhanced Cochlear Coverage and Hearing Preservation in High-Frequency Hearing Loss via Electric Acoustic Stimulation with Longer Electrode

Hidekane Yoshimura¹, Yutaka Takumi¹, Shin-ya Nishio², Shin-ichi Usami²

¹Department of Otorhinolaryngology - Head and Neck Surgery, Shinshu University School of Medicine, ²Department of Hearing Implant Sciences, Shinshu University School of Medicine

Electric acoustic stimulation (EAS) with longer electrodes can offer broader cochlear coverage and various types of maps in cases of high-frequency hearing loss. Combining less invasive surgery, flexible lateral-wall electrodes, and steroid administration permits deeper insertion with little or no surgical trauma, resulting in good preservation of hearing.