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Louisiana State University Health Sciences Center in Shreveport

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
Maciej T. Nogalski 1,2, Gary C.T. Chan 3, Emily V. Stevenson 1,2, Donna K. Collins-McMillen 1,2, Andrew D. Yurochko 1,2,4
1Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology, SUNY Upstate Medical University, 4Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center

The phagokinetic motility track assay is a method used to assess the movement of cells. Specifically, the assay measures chemokinesis (random cell motility) over time in a quantitative manner. The assay takes advantage of the ability of cells to create a measurable track of their movement on colloidal gold-coated coverslips.

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Medicine

Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice
Norman R. Harris 1, Megan N. Watts 1, Wendy Leskova 1
1Department of Molecular & Cellular Physiology, Louisiana State University Health Sciences Center

Intravital microscopy can be used in animals to visualize and measure retinal vascular diameters, bloodstream velocities, and total retinal blood flow.

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Biology

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
Luke A. White 1, Emily V. Stevenson 1, J. Winny Yun 1, Randa Eshaq 1, Norman R. Harris 1, David K. Mills 2, Alireza Minagar 3, Pierre-Olivier Couraud 4, J. Steven Alexander 1
1Molecular and Cellular Physiology, Louisiana State University Health Sciences Center in Shreveport, 2Biological Sciences, Louisiana Tech University, 3Neurology, Louisiana State University Health Sciences Center in Shreveport, 4Institut Cochin, Inserm U1016, Cnrs Umr8104, Université Paris Descartes

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.

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Behavior

Utilizing the Modified T-Maze to Assess Functional Memory Outcomes After Cardiac Arrest
Celeste Y. C. Wu 1,2, Francesca M. Lerner 4, Alexandre Couto e Silva 3, Harlee E. Possoit 1,2, Tsung-Han Hsieh 1,2, Jake T. Neumann 5, Alireza Minagar 1, Hung Wen Lin 1,2,3, Reggie H. C. Lee 1,2
1Department of Neurology, Louisiana State University Health Science Center, 2Center for Brain Health, Louisiana State University Health Science Center, 3Department of Cellular Biology and Anatomy, Louisiana State University Health Science Center, 4Department of Neurology, Cerebral Vascular Disease Research Laboratories, University of Miami Miller School of Medicine, 5Department of Biomedical Sciences, West Virginia School of Osteopathic Medicine

This protocol describes the use of a modified T-maze to evaluate functional learning/memory in asphyxia cardiac arrest-induced cerebral ischemia.

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