Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled ReceptorsMatthew R. Fleming 1, Steven M. Shamah 3, Leonard K. Kaczmarek 1,2
1Department of Pharmacology, Yale School of Medicine, 2Department of Cellular and Molecular Physiology, Yale School of Medicine, 3X-BODY Biosciences
Optical biosensor techniques can detect changes in mass near the plasma membrane in living cells and allow one to follow cellular responses in both individual cells and populations of cells. This protocol will describe detection of the modulation of potassium channels by G-protein coupled receptors in intact cells using this approach.
