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University of Basel and University Hospital Basel

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

Mathias Schmaler^*1, Maria A. S. Broggi^*1, Simona W. Rossi¹

¹Department of Biomedicine, Immunoregulation, University of Basel and University Hospital Basel

Tail-skin transplantation is a powerful model for studying T cell-dependent rejection and tolerance induction during allogeneic immune responses in mice. The advantages of this protocol are minor invasive surgery, and ease of monitoring with no need to sacrifice the recipient mouse.

Immunology and Infection

Isolation of Murine Lymph Node Stromal Cells

Maria A. S. Broggi^*1, Mathias Schmaler^*1, Nadège Lagarde¹, Simona W. Rossi¹

¹Department of Biomedicine, Immunoregulation, University of Basel and University Hospital Basel

Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.

Cancer Research

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Cinzia Donato^*1, Barbara M. Szczerba^*1, Manuel C. Scheidmann^*1, Francesc Castro-Giner^1,2, Nicola Aceto¹

¹Cancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, ²SIB Swiss Institute of Bioinformatics

Here, we present an integrated workflow to identify phenotypic and molecular features that characterize circulating tumor cells (CTCs). We combine live immunostaining and robotic micromanipulation of single and clustered CTCs with single cell-based techniques for downstream analysis and assessment of metastasis-seeding ability.