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Tokyo Women's Medical University

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

Takeshi Tomita^1,2, Katsuaki Ieguchi¹, Atsuko Deguchi¹, Morichika Takita¹, Fujiko Tsukahara¹, Sachie Hiratsuka^1,2, Yoshiro Maru¹

¹Department of Pharmacology, Tokyo Women's Medical University, ²Department of Biochemistry and Molecular Biology, School of Medicine, Shinshu University

This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.

Bioengineering

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Morito Sakuma¹, Yoshikazu Kumashiro², Masamichi Nakayama², Nobuyuki Tanaka², Yuji Haraguchi², Kazuo Umemura¹, Tatsuya Shimizu², Masayuki Yamato², Teruo Okano²

¹Department of Physics, Tokyo University of Science, ²Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University

Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.

Medicine

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Yuka Kato¹, Takanori Iwata², Kaoru Washio², Toshiyuki Yoshida², Hozue Kuroda², Shunichi Morikawa³, Mariko Hamada¹, Kazuki Ikura¹, Nobuyuki Kaibuchi², Masayuki Yamato², Teruo Okano², Yasuko Uchigata¹

¹Diabetic Center, Tokyo Women's Medical University School of Medicine, ²The Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, ³The Department of Anatomy and Developmental Biology, Tokyo Women's Medical University School of Medicine

Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.