Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response TestingAshley N. Gilbert 1, Rachael S. Shevin 4, Joshua C. Anderson 2, Catherine P. Langford 3, Nicholas Eustace 2, G. Yancey Gillespie 3, Raj Singh 4, Christopher D. Willey 2
1Biomedical Engineering, University of Alabama at Birmingham, 2Radiation Oncology, University of Alabama at Birmingham, 3Neurosurgery, University of Alabama at Birmingham, 4Vivo Biosciences, Inc.
Patient-derived xenografts of glioblastoma multiforme can be miniaturized into living microtumors using 3D human biogel culture system. This in vivo-like 3D tumor assay is suitable for drug response testing and molecular profiling, including kinomic analysis.
