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Mendel University in Brno

3 ARTICLES PUBLISHED IN JoVE

Chemistry

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Yazan Haddad^1,2, Simona Dostalova^1,2, Jiri Kudr^1,2, Ondrej Zitka^1,2, Zbynek Heger^1,2, Vojtech Adam^1,2

¹Department of Chemistry and Biochemistry, Mendel University in Brno, ²Central European Institute of Technology, Brno University of Technology

This protocol describes the synthesis of magnetic particles and evaluation of their DNA-binding properties via dynamic and electrophoretic light scattering. This method focuses on monitoring changes in particle size, their polydispersity and zeta potential of particle surface which play major role in binding of materials such as DNA.

Environment

Leaf Area Index Estimation Using Three Distinct Methods in Pure Deciduous Stands

Jakub Černý¹, Radek Pokorný², Pavel Haninec^2,3, Pavel Bednář¹

¹Forestry and Game Management Research Institute, Research Station at Opočno, ²Department of Silviculture, Mendel University in Brno, ³Department of Forest Botany, Dendrology and Geobiocenology, Mendel University in Brno

An accurate estimation of leaf area index (LAI) is crucial for many models of material and energy fluxes within plant ecosystems and between an ecosystem and the atmospheric boundary layer. Therefore, three methods (litter traps, needle technique, and PCA) for taking precise LAI measurements were in the presented protocol.

Environment

Field Measurement of Effective Leaf Area Index using Optical Device in Vegetation Canopy

Jakub Černý^1,2, Radek Pokorný²

¹Forestry and Game Management Research Institute, Research Station at Opočno, ²Department of Silviculture, Faculty of Forestry and Wood Technology, Mendel University in Brno

Fast and precise leaf area index (LAI) estimation in terrestrial ecosystems is crucial for a wide range of ecological studies and calibrating remote sensing products. Presented here is the protocol for using the new LP 110 optical device for taking ground-based in situ LAI measurements.