Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen SubstratesHitomi Fujisaki 1, Sugiko Futaki 2, Kazunori Mizuno 1, Shunji Hattori 1
1Nippi Research Institute of Biomatrix, 2Department of Anatomy and Cell Biology, Osaka Medical College
Here, we present a protocol for the preparation of two different forms of culture substrates utilizing type I collagen. Depending on how collagen is handled, collagen molecules either maintain two-dimensional, non-fibrous form or reassemble into three-dimensional, fibril form. Cell proliferation on type I collagen is drastically affected by fibril formation.
