A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia ColiSumana Venkat 1,2, Caroline Gregory 3, Kexin Meng 4, Qinglei Gan 1, Chenguang Fan 1,2
1Department of Chemistry and Biochemistry, University of Arkansas, 2Cell and Molecular Biology Program, University of Arkansas, 3Department of Biological Sciences, University of Arkansas, 4Fayetteville High School
Genetic code expansion serves as a powerful tool to study a wide range of biological processes, including protein acetylation. Here we demonstrate a facile protocol to exploit this technique for generating homogeneously acetylated proteins at specific sites in Escherichia coli cells.
