New South Wales Health Pathology
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
Murray C. Killingsworth 1,2,3,4, Yuri V. Bobryshev 3,4,5
1South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales Australia, 2School of Medicine, Western Sydney University, 3Correlative Microscopy Group, Ingham Institute for Applied Medical Research, 4Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service, New South Wales Health Pathology, 5School of Medical Sciences, Faculty of Medicine, University of New South Wales Australia
A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.
Biology
Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats via High Throughput Immunomagnetic Bead Separation
Maria Villalva 1, Sara Macphail 1, Yingshi Li 1, Beth Caruana 1
1New South Wales Health Statewide Biobank, New South Wales Health Pathology
This protocol details a high-throughput automation-compatible method to isolate human peripheral blood mononuclear cells for biobanking and other purposes.
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