Minimally Invasive Murine Laryngoscopy for Close-Up Imaging of Laryngeal Motion During Breathing and SwallowingTeresa E. Lever 1,2, Ashley Kloepper 1, Lauren Welby 3, Megan Haney 4, Shakera Fudge 4, Chase Seiller 5, Sophia Kington 2, Brayton Ballenger 5, Nicole L. Nichols 2
1Department of Otolaryngology - Head and Neck Surgery, University of Missouri School of Medicine, 2Department of Biomedical Sciences, University of Missouri College of Veterinary Medicine, 3Division of Otolaryngology - Head and Neck Surgery, Texas A&M Health Science Center College of Medicine - Baylor Scott & White, 4Department of Veterinary Pathobiology, University of Missouri College of Veterinary Medicine, 5Department of Medicine, University of Missouri School of Medicine
This protocol describes a serial transoral laryngoscopy approach for mice and rats that permits close-up, unobstructed video imaging of the larynx during breathing and swallowing using an optimized anesthetic regimen and finely tuned endoscopic manipulation techniques.
