Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During InfectionHye-Young Lee 1, So Eui Lee 1, Jongchan Woo 2, Doil Choi 1, Eunsook Park 1
1Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 2Department of Bioindustry and Bioresource Engineering, Sejong University
Fluorescent protein-based approaches to monitor effectors secreted by bacteria into host cells are challenging. This is due to the incompatibility between fluorescent proteins and the type-III secretion system. Here, an optimized split superfolder GFP system is used for visualization of effectors secreted by bacteria into the host plant cell.
