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Shengli Oilfield Central Hospital

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Zhiwei Xie^1,2,3, Jizhou Shi⁴, Min Zong³, Qiuping Xu¹, Chang Liu¹, Jie Wen¹, Qun Zhang¹, Panpan Liu¹, Guanyi Liu¹, Jing Guo^2,5, Xunwei Wu¹

¹Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ²Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ³Department of Stomatology, Shengli Oilfield Central Hospital, ⁴Department of Pediatric Surgery, Shengli Oilfield Central Hospital, ⁵Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.

Immunology and Infection

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Jin-Xin Lai^*1,2, Yu-Rong Qin^*1,3, Yi-Wen Liao^*4, Yu-Ting Si¹, Quan Yuan¹, Shi-Mei Huang², Yu-Rong Tang², Ji-Liang Wang², Liang Wang¹

¹Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, ²Department of Laboratory Medicine, Shengli Oilfield Central Hospital, ³Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, ⁴Dermatology Hospital, Southern Medical University

Various bacterial pathogens can cause respiratory tract infections and lead to serious health issues if not detected accurately and treated promptly. Rapid and accurate detection of these pathogens via loop-mediated isothermal amplification provides effective management and control of respiratory tract infections in clinical settings.