Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue SpecimensMichael Surace 1, Karma DaCosta 1, Anna Huntley 1, Weiguang Zhao 1, Christopher Bagnall 2, Charles Brown 1, Chichung Wang 3, Kristin Roman 3, Jennifer Cann 1, Arthur Lewis 2, Keith Steele 1, Marlon Rebelatto 1, Edwin R. Parra 4, Clifford C. Hoyt 3, Jaime Rodriguez-Canales 1
1Laboratory of Pathology, MedImmune, 2Laboratory of Pathology, MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology, University of Texas - MD Anderson Cancer Center
A detailed protocol for a six-marker multiplex immunofluorescence panel is optimized and performed, using an automated stainer for more consistent results and a shorter procedure time. This approach can be directly adapted by any laboratory for immuno-oncology studies.
