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University of Minnesota/ Agroinnova, University of Torino

2 ARTICLES PUBLISHED IN JoVE

Biology

Gibberella zeae Ascospore Production and Collection for Microarray Experiments.

¹Cereal Disease Laboratory, USDA, ²University of Minnesota/ Agroinnova, University of Torino, ³Cereal Disease Laboratory, University of Minnesota

To study the developmental processes of ascospores in Gibberella zeae, a procedure for collection under sterile conditions is filmed in order to generate the highest level of information for protocol description. This should facilitate the reproducibility of the experiment, a crucial aspect when full genome expression profile tests are implemented.

Biology

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Matias Pasquali¹, Frédéric Giraud¹, Jean Paul Lasserre¹, Sebastien Planchon¹, Lucien Hoffmann¹, Torsten Bohn¹, Jenny Renaut¹

¹Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox), Centre de Recherche Public-Gabriel Lippmann

Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.