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University of Science and Technology

6 ARTICLES PUBLISHED IN JoVE

Neuroscience

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

Mina Lee^1,2, Dongwook Kim^1,2, Hee-Sup Shin^1,2, Ho-Geun Sung³, Jee Hyun Choi^1,2

¹Center for Neural Science , Korea Institute of Science and Technology (KIST), ²Department of Neuroscience, University of Science and Technology, ³Fab Service Department, Korea Advanced Nano Fab Center

In this article, we described the surgery procedure and handling tips for implantation of ultra-thin polyimide-based microelectrode array (PBM-array) on the mouse skull for acquisition of high-density encephalography (EEG) in a mouse model.

Education

Magnetic Tweezers for the Measurement of Twist and Torque

Jan Lipfert¹, Mina Lee¹, Orkide Ordu¹, Jacob W. J. Kerssemakers¹, Nynke H. Dekker¹

¹Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology

Magnetic tweezers, a powerful single-molecule manipulation technique, can be adapted for the direct measurements of the twist (using a configuration called freely-orbiting magnetic tweezers) and torque (using a configuration termed magnetic torque tweezers) in biological macromolecules. Guidelines for performing such measurements are given, including applications to the study of DNA and associated nucleo-protein filaments.

Biochemistry

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Haseong Kim^*1, Gui Hwan Han^*1, Yaoyao Fu¹, Jongsik Gam², Seung Goo Lee^1,3

¹Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, ²College of Interdisciplinary & Creative Studies, Konyang University, ³Biosystems and Bioengineering Program, University of Science and Technology

This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

Chemistry

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a ¹²⁵I-labeled Azide Prosthetic Group

Jongho Jeon^1,2, Ha Eun Shim¹, Sajid Mushtaq^1,2, Mi Hee Choi¹, Sang Hyun Park^1,2, Dae Seong Choi¹, Beom-Su Jang^1,2

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, ²Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology

A detailed procedure for the synthesis of a ¹²⁵I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.

Neuroscience

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

Anvar Sariev^1,2, Jinho Chung¹, Dajung Jung^1,3, Farnaz Sharif^1,2, Ju-Young Lee^1,2, Soyoun Kim¹, Sebastien Royer^1,2

¹Center for Functional Connectomics, Korea Institute of Science and Technology, ²Biomedical department, KIST school, University of Science and Technology, ³Department of Biological Sciences, Korea Advanced Institute of Science and Technology

We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.

Engineering

An Efficient Method for Selective Desalination of Radioactive Iodine Anions by Using Gold Nanoparticles-Embedded Membrane Filter

Ha Eun Shim^*1,2, Sajid Mushtaq^*1,3, Jongho Jeon^1,3

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, ²Department of Chemistry, Kyungpook National University, ³Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology

An efficient method for the rapid and ion-selective desalination of radioactive iodine in several aqueous solutions is described by using gold nanoparticles-immobilized cellulose acetate membrane filters.