Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained MiceJulio E. Ayala 1, Deanna P. Bracy 2,3, Carlo Malabanan 3, Freyja D. James 2,3, Tasneem Ansari 3, Patrick T. Fueger 4, Owen P. McGuinness 2,3, David H. Wasserman 2,3
1Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology, Indiana University School of Medicine
The hyperinsulinemic-euglycemic clamp, or insulin clamp, is the gold standard for assessing insulin action in vivo. A method for performing insulin clamps in mice is described. This includes a method for arterial catheterization that permits experiments to be performed in conscious, unrestrained mice with minimal stress.
