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The University of New South Wales

10 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages
Kuldip Sidhu 1, Jaemin Kim 1, Methichit Chayosumrit 2, Sophia Dean 1, Perminder Sachdev 3
1Stem Cell Lab, School of Psychiatry, Faculty of Medicine, The University of New South Wales, 2Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University , 3Neuropsychiatric Institute, Prince of Wales Hospital

We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.

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Bioengineering

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
Siti Hawa Ngalim 1, Astrid Magenau 1, Ying Zhu 2, Lotte Tønnesen 1, Zoe Fairjones 1, J. Justin Gooding 2, Till Böcking 1, Katharina Gaus 1
1Centre for Vascular Research and Australian Centre for Nanomedicine, The University of New South Wales, 2School of Chemistry and Australian Centre for Nanomedicine, The University of New South Wales

A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.

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Engineering

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
Andrew Nattestad 1, Yuen Yap Cheng 2, Rowan W. MacQueen 2, Gordon G. Wallace 1, Timothy W. Schmidt 3
1ARC Centre of Excellence for Electromaterials Science (ACES), Intelligent Polymer Research Institute (IPRI), The University of Wollongong, 2School of Chemistry, The University of Sydney, 3School of Chemistry, The University of New South Wales

An integrated device, incorporating a dye-sensitized solar cell and triplet-triplet annihilation up-conversion unit was produced, affording enhanced light harvesting, from a wider section of the solar spectrum. Under modest irradiation levels a significantly enhanced response to low energy photons was demonstrated, yielding a record figure of merit for dye-sensitized solar cells.

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Neuroscience

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique
Huazheng Liang 1,2, Emma Schofield 1, George Paxinos 1,2
1Brain Structure and Function Group, Neuroscience Research Australia, 2School of Medical Sciences, The University of New South Wales

Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.

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JoVE Core

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Alexander F. Mason 1, Pall Thordarson 1
1School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales

This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.

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Chemistry

Facile Synthesis of Worm-like Micelles by Visible Light Mediated Dispersion Polymerization Using Photoredox Catalyst
Jonathan Yeow 1,2,3, Jiangtao Xu 1,2,3, Cyrille Boyer 1,2,3
1Centre for Advanced Macromolecular Design (CAMD), The University of New South Wales, 2Australian Centre for NanoMedicine (ACN), The University of New South Wales, 3School of Chemical Engineering, The University of New South Wales

This article describes a process for producing polymeric self-assembled nanoparticles using visible light mediated dispersion polymerization. Using low energy visible light to control the polymerization allows for the reproducible formation of self-assembled worm-like micelles at high solids content.

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Biology

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
Huazheng Liang *1,2, Bassem Akladios *1, Cesar P. Canales *1, Richard Francis 3, Edna H. Hardeman 1, Annemiek Beverdam 1
1The School of Medical Sciences, University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility, University of New South Wales Australia

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

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Biology

Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes
Amani Alghalayini 1,2, Lele Jiang 1, Xi Gu 3, Guan Heng Yeoh 3, Charles G. Cranfield 1,2, Victoria Timchenko 3, Bruce A. Cornell 2,4, Stella M. Valenzuela 1,2,5
1School of Life Sciences, University of Technology Sydney, 2ARC Research Hub for Integrated Devices for End-user Analysis at Low-levels (IDEAL), Faculty of Science, University of Technology Sydney, 3School of Mechanical and Manufacturing Engineering, The University of New South Wales, 4Surgical Diagnostics Pty Ltd., 5Institute for Biomedical Materials and Devices, University of Technology Sydney

This work outlines a protocol to achieve dynamic, non-invasive monitoring of heat transfer from laser-irradiated gold nanoparticles to tBLMs. The system combines impedance spectroscopy for the real-time measurement of conductance changes across the tBLMs, with a horizontally focused laser beam that drives gold nanoparticle illumination, for heat production.

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Chemistry

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization
Nathaniel Corrigan 1, Cyrille Boyer 1
1Cluster for Advanced Macromolecular Design, and Australian Centre for Nanomedicine, School of Chemical Engineering, University of New South Wales

The present protocol describes the digital light processing-based 3D printing of polymeric materials using type I photoinitiated reversible addition-fragmentation chain transfer polymerization and the subsequent in situ material post-functionalization via surface-mediated polymerization. Photoinduced 3D printing provides materials with independently tailored and spatially controlled bulk and interfacial properties.

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Medicine

Robotic Vagus-Sparing Total Gastrectomy for CDH1 Gene Mutation Treatment
Daniel L. Chan 1, Michael L. Talbot 1
1Saint George and Sutherland Clinical School, Faculty of Medicine, The University of New South Wales

This article focuses on robotic vagus-sparing total gastrectomy. The techniques and pitfalls of vagus preservation, sutured esophagojejunostomy, jejunal pouch formation, and Roux-en-Y reconstruction with a staple-stapled jejunojejunostomy are discussed.

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