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Feinberg School of Medicine, Northwestern University

3 ARTICLES PUBLISHED IN JoVE

Biology

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Wen Lu^*1, Urko del Castillo^*1,2, Vladimir I. Gelfand¹

¹Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, ²IKERBASQUE, Basque Foundation for Science

Drosophila S2 cells and cultured neurons are great systems for imaging of motor-driven organelle transport in vivo. Here we describe detailed protocols for culturing both cell types, their imaging and analysis of transport.

Chemistry

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Ha Yong Song^*1, Seong-Hoon Park^*2, Hong-Jun Kang¹, Athanassios Vassilopoulos¹

¹Laboratory for Molecular Cancer Biology, Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, ²Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University

This protocol describes the required steps to execute in vitro and in vivo deacetylation assays in order to establish the role of proteins as specific deacetylation substrates for sirtuins and further study the role of reversible - lysine acetylation as a post-translational modification.

Biochemistry

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Ye Han^*1, Kyle A. Lyman^*1, Matt Clutter², Gary E. Schiltz³, Quratul-Ain Ismail¹, Xiangying Cheng¹, Chi-Hao Luan⁴, Dane M. Chetkovich^1,5

¹Davee Department of Neurology and Clinical Neurosciences, Feinberg School of Medicine, Northwestern University, ²Center for Molecular Innovation and Drug Discovery, Northwestern University, ³Department of Pharmacology, Feinberg School of Medicine, Northwestern University, ⁴High Throughput Analysis Laboratory, Department of Molecular Biosciences, Northwestern University, ⁵Department of Physiology, Feinberg School of Medicine, Northwestern University

The interaction between HCN channels and their auxiliary subunit has been identified as a therapeutic target in Major Depressive Disorder. Here, a fluorescence polarization-based method for identifying small molecule inhibitors of this protein-protein interaction, is presented.