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European Research Center for Medical Imaging, Campus de la Timone

3 ARTICLES PUBLISHED IN JoVE

Medicine

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Keith K. Fenrich¹, Pascal Weber¹, Genevieve Rougon^1,2, Franck Debarbieux^1,2

¹Developmental Biology Institute of Marseille-Luminy (IBDML), Aix Marseille University, ²Campus de la Timone, European Research Center for Medical Imaging (CERIMED)

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

Medicine

An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy

Clément Ricard^1,2, Fabio Stanchi^1,3, Geneviève Rougon^1,2, Franck Debarbieux^1,2

¹Developmental Biology Institute of Marseille, Aix Marseille University, ²European Research Center for Medical Imaging, Campus de la Timone, ³Vesalius Research Center, KU Leuven Campus Gasthuisberg

We have established a cortical orthotopic glioblastoma model in mice for intravital two-photon microscopy that recapitulates the biophysical constraints normally at play during the growth of the tumor. A chronic glass window replacing the skull above the tumor enables the follow-up of the tumor progression over time by two-photon microscopy.

Cancer Research

Flexible Organic Electronic Devices for Pulsed Electric Field Therapy of Glioblastoma

Marie C. Lefevre¹, Attila Kaszas¹, Gerwin Dijk^1,2, Martin Baca¹, Olivier Baudino², Bastien Marchiori², Loig Kergoat², David Moreau¹, Franck Debarbieux^3,4,5, Rodney P. O'Connor¹

¹Centre CMP, Bioelectronics Department, Mines Saint-Etienne, ²Panaxium SAS, ³Institut Universitaire de France, ⁴Institut des Neurosciences de la Timone (UMR7289), CNRS, Aix-Marseille Université, ⁵Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université

This work describes the development of flexible interdigitated electrodes for implementation in 3D brain tumor models, namely, in vitro culture, in ovo model, and in vivo murine model. The proposed method can be used to evaluate the effects of pulsed electric fields on tumors at different levels of complexity.