Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated TransportYves Molino 1, Françoise Jabès 1, Emmanuelle Lacassagne 2, Nicolas Gaudin 2, Michel Khrestchatisky 2
1VECT-HORUS SAS, 2Aix-Marseille Université, CNRS, NICN UMR 7259
The aim of the present study was to validate the reproducibility of an in vitro BBB model involving a rat syngeneic co-culture of endothelial cells and astrocytes. The endothelial cell monolayer presented high TEER and low LY permeability. Expression of specific TJ proteins, functional responses to inflammation and functionality of transporters and receptors were assessed.
