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Mount Sinai Medical Center

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy

Rachel Lubong Sabado¹, Elizabeth Miller², Meredith Spadaccia¹, Isabelita Vengco¹, Farah Hasan¹, Nina Bhardwaj¹

¹Cancer Institute, NYU Langone Medical Center, ²Infectious Diseases, NYU Langone Medical Center

The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.

Immunology and Infection

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Melissa V. Fernandez¹, Elizabeth A. Miller², Nina Bhardwaj³

¹Department of Pathology, New York University School of Medicine, ²Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, ³Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β expression.

Medicine

Focused Assessment with Sonography for Trauma (FAST) Exam: Image Acquisition

John D. Ritchie¹, Charles N. Trujillo², David L. Convissar³, William Shihao Lao⁴, Sean Montgomery⁵, Yuriy S. Bronshteyn⁶

¹Trauma, Acute, and Critical Care Surgery, Duke University Hospital, ²Mount Sinai Medical Center, ³Department of Anesthesiology, Massachusetts General Hospital, ⁴Acute Care Surgery, Atrium Health, ⁵Department of Surgery, Duke University School of Medicine, ⁶Department of Anesthesiology, Duke University School of Medicine, Duke University Health System, Durham Veterans Health Administration

The Focused Assessment with Sonography for Trauma (FAST) exam is a diagnostic point-of-care ultrasound examination used to screen for the presence of free fluid in the pericardium and peritoneum. Indications, techniques, and pitfalls of the procedure are discussed in this article.