Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained RatsJeffrey D. Moore 1, Martin Deschênes 2, David Kleinfeld 1
1Department of Physics 0374, University of California, San Diego, 2Department of Psychiatry and Neuroscience, Centre de Recherche de l’Université Laval Robert-Giffard
This protocol describes the design and surgical implantation of a head-restraining mechanism to monitor neuronal activity in sub-cortical brain structures in alert rats. It delineates procedures to isolate single neurons in the juxtacellular configuration and to efficiently identify their anatomical locations.
