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Sloan-Kettering Institute for Cancer Research

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Nadja Zeltner¹, Fabien G. Lafaille², Faranak Fattahi¹, Lorenz Studer¹

¹Developmental Biology, Center for Stem Cell Biology, Sloan-Kettering Institute for Cancer Research, ²St. Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University

Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.

Developmental Biology

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

Scott J. Callahan^1,2, Yvonne Mica³, Lorenz Studer¹

¹The Center for Stem Cell Biology, Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, ²Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School, Sloan-Kettering Institute for Cancer Research, ³Thermo Fisher Scientific

This work describes an in vitro differentiation protocol to produce pigmented, mature melanocytes from human pluripotent stem cells via a neural crest and melanoblast intermediate stage using a feeder-free, 25 day protocol.

Developmental Biology

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Hsueh Fu Wu^1,2, Nadja Zeltner^1,2,3

¹Center for Molecular Medicine, University of Georgia, ²Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences, University of Georgia, ³Department of Cellular Biology, Franklin College of Arts and Sciences, University of Georgia

In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.