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The Children's Hospital of Philadelphia, University of Pennsylvania

3 ARTICLES PUBLISHED IN JoVE

Medicine

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

Ilia Fishbein¹, Scott P. Forbes¹, Richard F. Adamo¹, Michael Chorny¹, Robert J. Levy¹, Ivan S. Alferiev¹

¹Department of Pediatrics, Division of Cardiology, The Children's Hospital of Philadelphia, University of Pennsylvania

These studies report on reversible attachment of adenoviral gene vectors to coatless metal surfaces of stents and model mesh disks. Sustained release of transduction-competent viral particles contingent upon hydrolysis of cross-linkers used for vector immobilization results in a durable site-specific transgene expression in vascular cells and in stented arteries.

Bioengineering

The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

Joshua B. Slee^1,2, Ivan S. Alferiev^1,2, Robert J. Levy^1,2, Stanley J. Stachelek^1,2

¹Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia, ²University of Pennsylvania Perelman School of Medicine

Blood exposure to polymeric blood conduits initiates the foreign body reaction that has been implicated in clinical complications. Here, the Chandler Loop Apparatus, an experimental tool mimicking blood perfusion through these conduits, is described. Appendage of recombinant CD47 results in decreased evidence of the foreign body reaction on these conduits.

Bioengineering

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

Vaishali V. Inamdar¹, Emmett G. Fitzpatrick¹, Ivan S. Alferiev^1,2, Robert J. Levy^1,2, Stanley J. Stachelek^*1,2, Ilia Fishbein^*1,2

¹The Children's Hospital of Philadelphia, ²Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania

Presented here is a protocol for appending peptide CD47 (pepCD47) to metal stents using polybisphosphonate chemistry. Functionalization of metal stents using pepCD47 prevents the attachment and activation of inflammatory cells thus improving their biocompatibility.