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Shriners Hospitals for Children - Northern California

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Dennis J. Wu¹, Neha Dixit¹, Erika Suzuki¹, Thanh Nguyen², Hyun Seock Shin¹, Jack Davis², Emanual Maverakis³, Iannis E. Adamopoulos^1,2

¹Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, ²Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children - Northern California, ³Department of Dermatology, University of California, Davis

Differentiation of precursor cells into osteoclasts is regulated by cytokines and growth factors. Here, a novel gene transfer technique for differentiation of osteoclasts in vivo and cell culture protocols for differentiating precursor cells into osteoclasts in vitro as a method to study the effects of cytokines on osteoclastogenesis are described.

Biology

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

Thanh Nguyen¹, Sang Joon Ahn², Richard West³, Ibra S. Fancher¹

¹Department of Kinesiology and Applied Physiology, College of Health Sciences, University of Delaware, ²Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, ³University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute, University of Delaware

This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.