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San José State University

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Joy A. Franco¹, Heidi E. Kloefkorn², Shawn Hochman³, Katherine A. Wilkinson⁴

¹Department of Mechanical Engineering, San José State University, ²J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, ³Department of Physiology, Emory University School of Medicine, ⁴Department of Biological Sciences, San José State University

Muscle sensory neurons are involved in proprioceptor signaling and also report on metabolic state and injury related events. We describe an adult mouse in vitro muscle-nerve preparation for studies on stretch-activated muscle afferents.

JoVE Journal

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device

Hoi Yan Ko¹, Kevin Dann¹, Liat Rosenfeld¹

¹Department of Chemical Engineering, San José State University

We present a protocol to study the formation of hydrates in the presence of nonionic surfactants on the interface of a water droplet submerged in cyclopentane. The protocol consists of building a low-cost, programmable, temperature regulator. The temperature control system is combined with visualization techniques and internal pressure measurements.

Biology

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

Laura Martins¹, Ramy Wong¹, Fredrick J. Larabee¹, Bree K. Grillo-Hill¹

¹Department of Biological Sciences, San José State University

Here, we present a rapid, low-cost, workflow for high-resolution imaging of adult Drosophila eyes to quantify patterning and growth defects. We describe our protocol for sample preparation by point-mounting, high-resolution image acquisition, and image analysis.