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Institute of Botany and Microbiology, VIB, KU Leuven

1 ARTICLES PUBLISHED IN JoVE

Medicine

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

Soňa Kucharíková¹, Greetje Vande Velde², Uwe Himmelreich², Patrick Van Dijck¹

¹Department of Molecular Microbiology, Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, VIB, KU Leuven, ²Biomedical MRI Unit/ MoSAIC, Department of Imaging & Pathology, KU Leuven

We present an experimental procedure of Candida albicans biofilm development in a mouse subcutaneous model. Fungal biofilms were quantified by determining the number of colony forming units and by a non-invasive bioluminescence imaging, where the amount of light that is produced corresponds with the number of viable cells.