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Biology

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
Audrey Chabrat *1,2, Hélène Doucet-Beaupré *1,2, Martin Lévesque 1,2
1Department of Psychiatry and Neurosciences, Faculty of Medicine, Université Laval, 2Centre de recherche de l'Institut universitaire en santé mentale de Québec

Gene expression analysis of a subset of cells in a specific tissue represents a major challenge. This article describes how to isolate high-quality total RNA from a specific cell population by combining a rapid immunolabeling method with laser capture microdissection.

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