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Chang Gung University College of Medicine

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
Shiun Chang 1, Li-Mei Pai 2,3, Chien-Kuo Lee 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Biochemistry, Chang Gung University College of Medicine, 3Molecular Medicine Research Center, Chang Gung University College of Medicine

In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.

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