In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell RemodelingWinston Elliott 1, Devon Scott-Drechsel 2, Wei Tan 1
1Department of Mechanical Engineering, University of Colorado at Boulder, 2Product Development, Covidien, Ltd.
This protocol replicates physiological or pathological blood flow in vitro to aid in determining cell response in disease pathologies. By introducing a pressure damping chamber downstream of a blood pump, blood flow across the vasculature can be recapitulated and imposed on a monolayer of vascular endothelium or a mimetic co-culture.
