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UCSD Stem Cell Program, University of California, San Diego

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

¹Department of Dermatology, UCSD Stem Cell Program, University of California, San Diego, ²Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego

The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.