Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In VivoDorota N. Komar 1, Alfonso Mouriz 1, José A. Jarillo 1, Manuel Piñeiro 1
1Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid
Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.
