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HHMI Janelia Research Campus

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Jaroslav Icha^*1, Christopher Schmied^*1, Jaydeep Sidhaye¹, Pavel Tomancak¹, Stephan Preibisch^1,2,3, Caren Norden¹

¹Max Planck Institute of Molecular Cell Biology and Genetics, ²HHMI Janelia Research Campus, ³Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

Immunology and Infection

Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

James J. Faust^1,2, Wayne Christenson^3,4,5, Kyle Doudrick⁶, John Heddleston⁷, Teng-Leong Chew⁷, Marko Lampe⁸, Arnat Balabiyev^1,2, Robert Ros^3,4,5, Tatiana P. Ugarova^1,2

¹Center for Metabolic and Vascular Biology, Mayo Clinic, ²Molecular and Cellular Biosciences, School of Life Sciences, Arizona State University, ³Department of Physics, Arizona State University, ⁴Center for Biological Physics, Arizona State University, ⁵Biodesign Institute, Arizona State University, ⁶Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, ⁷Advanced Imaging Center, HHMI Janelia Research Campus, ⁸Advanced Light Microscopy Facility, European Molecular Biology Laboratory

This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.