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Berlin Institute of Medical Systems Biology of the Max Delbrück Center

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Jaroslav Icha^*1, Christopher Schmied^*1, Jaydeep Sidhaye¹, Pavel Tomancak¹, Stephan Preibisch^1,2,3, Caren Norden¹

¹Max Planck Institute of Molecular Cell Biology and Genetics, ²HHMI Janelia Research Campus, ³Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.