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National Taipei University of Technology

3 ARTICLES PUBLISHED IN JoVE

Biochemistry

Preparation of Homogeneous MALDI Samples for Quantitative Applications

Yu-Meng Ou^1,2, Chien-Wei Tsao^1,3, Yin-Hung Lai¹, Hsun Lee¹, Huan-Tsung Chang², Yi-Sheng Wang¹

¹Genomic Research Center, Academia Sinica, ²Department of Chemistry, National Taiwan University, ³Department of Chemical Engineering and Biotechnology, National Taipei University of Technology

A protocol for reducing spatial heterogeneities of ion signals in MALDI mass spectrometry by regulating substrate temperature during sample drying processes is demonstrated.

Bioengineering

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Hua-De Gao^1,2, Pounraj Thanasekaran¹, Tzu-Ho Chen^1,2, Yu-Hsu Chang³, Yu-Ju Chen^1,2, Hsien-Ming Lee¹

¹Institute of Chemistry, Academia Sinica, ²Department of Chemistry, National Taiwan University, ³Department of Materials and Mineral Resources Engineering, National Taipei University of Technology

In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol.

Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium

Chun Hsiung^*1,2, Yu-Ting Liu^*1,2, Chen-Ying Su³, Chung-Chuan Hsiung^4,5, Kuo-Hsuan Hung^2,6,7, Lung-Kun Yeh^2,6

¹Department of Education, Chang Gung Memorial Hospital Linkou, ²School of Medicine, Chang Gung University, ³Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, ⁴Cell Therapy Center, Tri-Service General Hospital, ⁵Department of General Surgery, Bo-Ai Clinic, ⁶Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, ⁷Institute of Clinical Medicine, National Yang Ming Chiao Tung University

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.