SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected CellsCollins Cheruiyot 1, Zemplen Pataki 1, Robert Williams 1, Bharat Ramratnam 2,3, Ming Li 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University
Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.
