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Ben Gurion University of the Negev

3 ARTICLES PUBLISHED IN JoVE

Behavior

How to Use the H1 Deep Transcranial Magnetic Stimulation Coil for Conditions Other than Depression

Aron Tendler¹, Yiftach Roth¹, Noam Barnea-Ygael², Abraham Zangen²

¹Brainsway LTD, ²Department of Life Sciences, Ben Gurion University of the Negev

The H1 deep transcranial magnetic stimulation coil is FDA-cleared for the treatment of depression. We demonstrate how to utilize the H1 for other conditions, such as auditory hallucinations and PTSD, by moving the helmet to different locations over the subject's skull.

Biology

Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations

Grace A. Snyder¹, William E. Browne², Nikki Traylor-Knowles^*1, Benyamin Rosental^*3

¹Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, University of Miami, ²Department of Biology, University of Miami, ³The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center, Ben Gurion University of the Negev

Corals create biodiverse ecosystems important for both humans and marine organisms. However, we still do not understand the full potential and function of many coral cells. Here, we present a protocol developed for the isolation, labeling, and separation of stony coral cell populations.

Bioengineering

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Sakshi Choudhary¹, Gefen Livne¹, Shachar Gat¹, Anne Bernheim-Groswasser^1,2

¹Department of Chemical Engineering, Ben Gurion University of the Negev, ²Department of Chemical Engineering, Ilse Kats Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev

In this work, an in vitro reconstitution approach is employed to study the poroelasticity of actomyosin gels under controlled conditions. The dynamics of the actomyosin gel and the embedded solvent are quantified, through which the network poroelasticity is demonstrated. We also discuss the experimental challenges, common pitfalls, and relevance to cell cytoskeleton mechanics.