In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell ConnectivityCarlos G. Briz 1, Marta Navarrete 2, José A. Esteban 2, Marta Nieto 1
1Department for Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), 2Molecular Neurobiology Department, Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas (CSIC-UAM)
This manuscript provides protocols that use in utero electroporation (IUE) to describe the structural connectivity of neurons at the single-cell level and the excitability of fluorescently labeled neurons. Histology is used to characterize dendritic and axonal projections. Whole-cell recording in acute slices is used to investigate excitability.
