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CNRS, UPMC, UMR 7622, INSERM ERL U1156

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Estelle Hirsinger¹, João Emanuel Carvalho², Christine Chevalier^1,3, Georges Lutfalla⁴, Jean-François Nicolas¹, Nadine Peyriéras⁵, Michael Schubert²

¹Département de Biologie du Développement et Cellules Souches, Institut Pasteur, ²Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, ³Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, ⁴Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, ⁵Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard

We report here the robust and efficient expression of fluorescent proteins after mRNA injection into unfertilized oocytes of Branchiostoma lanceolatum. The development of the microinjection technique in this basal chordate will pave the way for far-reaching technical innovations in this emerging model system, including in vivo imaging and gene-specific manipulations.

Developmental Biology

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Estelle Hirsinger^1,3, Ben Steventon^1,2

¹Department of Developmental and Stem Cell Biology, Institut Pasteur, ²Department of Genetics, University of Cambridge, ³IBPS- Laboratoire de Biologie du Developpement (LBD), CNRS, UPMC, UMR 7622, INSERM ERL U1156

Here, we present a versatile mounting method that allows for the long-term time-lapse imaging of the posterior body development of live zebrafish embryos without perturbing normal development.