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Lombardi Comprehensive Cancer Center

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

Sung-Hyeok Hong^*1, Jason U. Tilan^*2,3, Susana Galli¹, Rachel Acree³, Katherine Connors⁴, Akanksha Mahajan¹, Larissa Wietlisbach³, Taylor Polk³, Ewa Izycka-Swieszewska⁵, Yi-Chien Lee⁶, Luciane R. Cavalli⁶, Olga C. Rodriguez⁶, Chris Albanese^6,7, Joanna B. Kitlinska¹

¹Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, ²Department of Nursing, Georgetown University, School of Nursing and Health Studies, ³Department of Human Science, Georgetown University, School of Nursing and Health Studies, ⁴School of Medicine, Georgetown University Medical Center, ⁵Department of Pathology and Neuropathology, Medical University of Gdańsk, ⁶Department of Oncology, Georgetown University Medical Center, ⁷Department of Pathology, Georgetown University Medical Center

This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.

Cancer Research

A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation

Lucas Tricoli¹, Deborah L. Berry², Chris Albanese¹

¹Department of Oncology, Lombardi Comprehensive Cancer Center, ²Department of Oncology, Georgetown University Medical Center

Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.