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Oncomedic Incorporation, Ciudad de México

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Orlando Santillán¹, Miguel A. Ramírez-Romero², Guillermo Dávila³

¹Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, ²Oncomedic Incorporation, Ciudad de México, ³Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México

Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI), a protocol based on the insertion of restriction enzyme sites into synonym DNA sequences and functional plasmid recovery. This protocol is a fast and low-cost method for fusing protein-coding genes.