Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug ResponseMattie J. Casey *1, Katarzyna Modzelewska *1, Daniela Anderson 1, James Goodman 1, Elena F. Boer 1, Laura Jimenez 1, Douglas Grossman 1,2, Rodney A. Stewart 1
1Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, 2Department of Dermatology, University of Utah Health Sciences Center, Salt Lake City
The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.
