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University of the Basque Country, UPV/EHU

1 ARTICLES PUBLISHED IN JoVE

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Aintzane Apraiz *1, Jone Mitxelena *2,3, Ana Zubiaga 2
1Department of Cell Biology and Histology, University of the Basque Country, UPV/EHU, 2Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, 3Department of Molecular Mechanisms of Disease, University of Zurich

We report two cell synchronization protocols that provide a context for studying events related to specific phases of the cell cycle. We show that this approach is useful for analyzing the regulation of specific genes in an unperturbed cell cycle or upon exposure to agents affecting the cell cycle.

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