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Institut du Cerveau et de la Moelle épinière, ICM

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Elodie Martin^1,2,3, Mohamed El-Behi^1,2,3, Bertrand Fontaine^1,2,3,4, Cecile Delarasse^1,2,3

¹Inserm U 1227, CNRS UMR 7225, ²Sorbonne Universités, UPMC, University of Paris, ³Institut du Cerveau et de la Moelle épinière, ICM, ⁴AP-HP, Hôpital de la Pitié Salpêtrière;

This protocol provides an analysis of the macrophage subpopulations in the adult mouse central nervous system by flow cytometry and is helpful for the study of multiple markers expressed by these cells.

Neuroscience

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Melina Thetiot¹, Rémi Ronzano¹, Marie-Stéphane Aigrot¹, Catherine Lubetzki¹, Anne Desmazières¹

¹Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.

Neuroscience

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Elisa Mazuir¹, Anne-Laure Dubessy^1,2, Loane Wallon^1,3, Marie-Stéphane Aigrot¹, Catherine Lubetzki^1,2, Nathalie Sol-Foulon¹

¹Sorbonne Université, Inserm, CNRS, UMR7225, Institut du Cerveau et de la Moelle épinière, ICM, ²Assistance Publique-Hôpitaux de Paris, GH Pitié Salpêtrière, ³Ecole Supérieure des Techniques de Biologie Appliquée

Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.