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Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Yaqian Li^*1,2, Xiaojun Yan^*1, Wei Liu^*1, Lyu Zhou^1,3, Zhifeng You¹, Yanan Du^1,2

¹Department of Biomedical Engineering, School of Medicine, Tsinghua University, ²Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, ³School of Life Sciences, Tsinghua University

This protocol describes the fabrication of elastic 3D macroporous microcryogels by integrating microfabrication with cryogelation technology. Upon loading with cells, 3D microtissues are generated, which can be readily injected in vivo to facilitate regenerative therapy or assembled into arrays for in vitro high-throughput drug screening.

Medicine

Establishing a Silicosis Rat Model via Exposure of Whole-Body to Respirable Silica

Fuyu Jin¹, Yaqian Li¹, Tian Li¹, Xinyu Yang¹, Wenchen Cai¹, Shifeng Li¹, Xuemin Gao¹, Fang Yang¹, Hong Xu¹, Heliang Liu¹

¹Hebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology

This study describes a technique to establish a silicosis rat model with the inhalation of silica through the whole body in an inhalation chamber. The rats with silicosis could closely mimic the pathological process of human silicosis in an easy, cost-effective manner with good repeatability.