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German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Caghan Kizil¹, Anne Iltzsche¹, Jan Kaslin¹, Michael Brand¹

¹DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

In this article, we demonstrate a method for manipulation of gene expression in the ventricular cells of the adult zebrafish telencephalon using antisense morpholino oligonucleotides. We present this method as an efficient and quick protocol that can be used for functional studies in the adult vertebrate brain.

Neuroscience

Modeling Amyloid-β42 Toxicity and Neurodegeneration in Adult Zebrafish Brain

Prabesh Bhattarai¹, Alvin Kuriakose Thomas², Mehmet Ilyas Cosacak¹, Christos Papadimitriou¹, Violeta Mashkaryan³, Yixin Zhang², Caghan Kizil^1,3

¹German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association, ²B CUBE, Center for Molecular Bioengineering, Technische Universität Dresden, ³Center for Regenerative Therapies Dresden (CRTD), TU Dresden

This protocol describes the synthesis, characterization, and injection of monomeric amyloid-β42 peptides for generating amyloid toxicity in adult zebrafish to establish an Alzheimer's disease model, followed by histological analyses and detection of aggregations.