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McGill University and Génome Québec Innovation Centre

3 ARTICLES PUBLISHED IN JoVE

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Biology

The Microfluidic Probe: Operation and Use for Localized Surface Processing
Cecile M. Perrault 1, Mohammad A. Qasaimeh 1, David Juncker 1
1Department of Biomedical Engineering, McGill University

In this video we present the microfluidic probe1 (MFP). We explain in detail how to assemble the MFP, mount it atop an inverted microscope, and align it relative to the substrate surface, and finally show how to use it to process a substrate surface immersed in a buffer solution.

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Chemistry

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
Huiyan Li 1, Robert Popp 1, Bjorn Frohlich 1, Michael X. Chen 2, Christoph H. Borchers 1,3,4,5
1University of Victoria-Genome BC Proteomics Centre, 2Jewish General Hospital, McGill University, 3Dept of Biochemistry and Microbiology, University of Victoria, 4Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital, McGill University, 5Gerald Bronfman Department of Oncology, Jewish General Hospital

A protocol for the protein quantification in complex biological fluids using automated immuno-MALDI (iMALDI) technology is presented.

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Bioengineering

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
Huiyan Li 1,2, Sebastien Bergeron 3, Heidi Larkin 1,2,3, David Juncker 1,2
1McGill University and Génome Québec Innovation Centre, 2Biomedical Engineering Department, McGill University, 3Parallex BioAssays Inc.

We demonstrate a snap chip technology for performing cross-reactivity-free multiplexed sandwich immunoassays by simply snapping two slides. A snap apparatus is used for reliably transferring reagents from microarray-to-microarray. The snap chip can be used for any biochemical reactions requiring colocalization of different reagents without cross-contamination.

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