Tennessee Valley Healthcare System
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
piggyBac Transposon System Modification of Primary Human T Cells
Sunandan Saha 1,2, Yozo Nakazawa 3, Leslie E. Huye 4,5, Joseph E. Doherty 2,6, Daniel L. Galvan 2, Cliona M. Rooney 4,5,7, Matthew H. Wilson 1,2,4,8
1Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , 2Department of Medicine, Division of Nephrology, Baylor College of Medicine , 3Department of Immunology and Pathology, Shinshu University School of Medicine, 4Center for Cell and Gene Therapy, Baylor College of Medicine , 5Department of Pediatrics, Baylor College of Medicine , 6Program in Cell and Molecular Biology, Baylor College of Medicine , 7Department of Molecular Virology and Microbiology, Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center
We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.
Biology
Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
Lauren E. Woodard 1,2,3, Richard C. Welch 2, Felisha M. Williams 2, Wentian Luo 2, Jizhong Cheng 3, Matthew H. Wilson 1,2,3
1Department of Veterans Affairs, Tennessee Valley Healthcare System, 2Departments of Medicine and Pharmacology, Vanderbilt University Medical Center, 3Department of Medicine, Baylor University College of Medicine
This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.
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