Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient SourcesAudrey Bloem 1, Stephanie Rollero 2, Pauline Seguinot 1, Lucie Crépin 3, Marc Perez 1, Christian Picou 1, Carole Camarasa 1
1UMR SPO, INRA, SupAgroM, Université de Montpellier, 2IWBT Stellenbosch University, 3PILI, Toulouse White Biotechnology
This protocol describes an experimental procedure to quantitatively and comprehensively investigate the metabolism of multiple nutrient sources. This workflow, based on a combination of isotopic tracer experiments and an analytical procedure, allows the fate of consumed nutrients and the metabolic origin of molecules synthetized by microorganisms to be determined.
